Applied Biosystems 7500 User Manual

Chapter 5 Performing the Background Calibration and Optical Calibration
Troubleshooting
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 65
Notes
5
4. Locate the contaminated well position(s) by selecting
successively fewer wells in the plate document.
The items that follow show an example of how to determine
the location of a contaminated well.
a. In the Spectra tab, select columns 1-6. The raw data from
the selected wells does not include the irregular peak.
Therefore, the contaminated well must be in columns 7-12.
b. Select columns 7-9. The raw data from the selected wells
includes the irregular peak. The contaminated well must be
in columns 7-9.
c. Select wells in row E and below in columns 7-9. The raw
data from the selected wells does not include the irregular
peak. The contaminated well must be in the first four wells
of columns 7-9.
d. Finally, by selecting each of the wells from the first four
wells of columns 7-9, you can determine the location of the
contaminated well (B9).
5. Repeat step 4 until you identify the location of each
contaminated well.
Troubleshooting – Background Calibration
Contaminated well
is not displayed
Contaminated
well
Contaminated
well is not
displayed
Contaminated
well
Contaminated
well

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)