EasyManuals Logo

Applied Biosystems 7500 User Manual

Applied Biosystems 7500
174 pages
To Next Page IconTo Next Page
To Next Page IconTo Next Page
To Previous Page IconTo Previous Page
To Previous Page IconTo Previous Page
Page #91 background imageLoading...
Page #91 background image
Chapter 5 Performing the Background Calibration and Optical Calibration
Troubleshooting
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 65
Notes
5
4. Locate the contaminated well position(s) by selecting
successively fewer wells in the plate document.
The items that follow show an example of how to determine
the location of a contaminated well.
a. In the Spectra tab, select columns 1-6. The raw data from
the selected wells does not include the irregular peak.
Therefore, the contaminated well must be in columns 7-12.
b. Select columns 7-9. The raw data from the selected wells
includes the irregular peak. The contaminated well must be
in columns 7-9.
c. Select wells in row E and below in columns 7-9. The raw
data from the selected wells does not include the irregular
peak. The contaminated well must be in the first four wells
of columns 7-9.
d. Finally, by selecting each of the wells from the first four
wells of columns 7-9, you can determine the location of the
contaminated well (B9).
5. Repeat step 4 until you identify the location of each
contaminated well.
Troubleshooting – Background Calibration
Contaminated well
is not displayed
Contaminated
well
Contaminated
well is not
displayed
Contaminated
well
Contaminated
well

Table of Contents

Other manuals for Applied Biosystems 7500

Questions and Answers:

Question and Answer IconNeed help?

Do you have a question about the Applied Biosystems 7500 and is the answer not in the manual?

Applied Biosystems 7500 Specifications

General IconGeneral
Detection MethodFluorescence
Sample Capacity96-well plate
Thermal CyclerYes
Temperature Uniformity±0.5°C
TypeReal-Time PCR System
Thermal Accuracy±0.25°C
Detection Channels5
Weight45 kg
Power Requirements100-240 V, 50/60 Hz
Dynamic Range9 logs
Reaction Volume Range10-100 µL
Temperature Range4°C to 100°C
Thermal Cycler Block96-well
SoftwareSDS Software
DetectionMultiplex (up to 5 colors)

Related product manuals