PN 177196BB
7-19
DATA MANAGEMENT AND REVIEW
DATA ANALYSIS REVIEW - WBC PARAMETERS
7
DiffPlot Thresholds
1. Volume and absorbance thresholds are used to detect shifting populations.
a. Most of the population partition thresholds are fixed and give the limits of the
morphological normality of leukocytes.
b. Changes in the morphology of a population are expressed on the DiffPlot by a
shifting of the corresponding population.
2. When populations in DiffPlot exceed limits set for that region:
a. A review R-flag occurs on the DIFF parameter related to that region.
b. Either DiffPlot and Histogram flags or Analytical Alarms occur to indicate the area
within the DiffPlot that is affected.
c. If an R-flag occurs on a DIFF parameter, further investigate the result.
3. Ten different flags may occur related to the position of the populations within the
DiffPlot:
Immature White Blood Cells on the DiffPlot
Blasts,
Promyelocytes
typical Lymphs,
Blasts
Abnormal
Lymphs
Myelocytes,
Metamyelocytes,
Immature Neutrophil
Bands
Debris,
NRBCs,
Lyse Resistant RBCs,
Plt Aggregates
Immature Eosinophil
Agranular Eosinophil
Atypical Neutrophils
VOLUME
ABSORBANCE
Eosinophils
Neutrophils
Monocytes
Lymphs
r SL (small lymphocytes) r LN (lower neutrophil)
r SL1 (small lymphocytes 1) r UN (upper neutrophil)
r NL (neutrophil/lymphocyte) r NE (neutrophil/eosinophil)
r MN (monocyte/neutrophil) r ATL (atypical lymphocytes)
r UM (upper monocyte) r IMM (immature cells)