PN 177196BB
8-10
SYSTEM DIAGNOSTICS AND MAINTENANCE
RECOGNIZING NORMAL INSTRUMENT OPERATION - CYCLE DESCRIPTION
Preparing for Sample Processing
1. After the cycle is initiated:
a. The sampling probe moves to the pierce
position.
b. The sampling probe move down to the
vial, the needle pierces the cap, and the
sampling probe moves to the correct level
in the vial to aspirate a sample of blood.
1) 53 µL in the CBC/DIFF mode.
2) 30 µL in the CBC mode.
2. All the baths drain.
Rinsing the Probe Exterior After Aspiration
1. The horizontal traverse assembly positions the
sampling probe over the RINSE bath.
2. The 3 µL sample aliquot at the tip of the
sampling probe is discarded into the RINSE
bath as the exterior of the sampling probe is
rinsed. Discarding this aliquot helps ensure
sample integrity.
3. Dispensing an aliquot of the sample into the
RINSE bath begins the sample partitioning for
the sequential dilution system.
a. At this point you may want to refer to
Heading 7.2, DATA ANALYSIS - AN
OVERVIEW OF THE DILUTIONS AND
MEASUREMENTS, in this Training Guide
and review the information under
Preparing the Dilutions for Sample
Analysis.
b. If you are letting the instrument cycle as
you explain the flow, you may want to have
the trainee focus on the sampling probe for
one cycle as it dispenses sample.
7616225D
DIL 1
HGB
DIFF RBC BASO
WBC
RINSE
DIL 1
HGB
DIFF
RBC
BASO
WBC
RINSE
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