PN 177196BB
8-11
SYSTEM DIAGNOSTICS AND MAINTENANCE
RECOGNIZING NORMAL INSTRUMENT OPERATION - CYCLE DESCRIPTION
8
1. Each aliquotted sample is delivered to its
appropriate bath using a tangential flow of
reagent which mixes the diluted sample and
minimizes viscosity problems.
2. To create this tangential flow:
a. The reagent delivery port is positioned so
that reagent is delivered tangentially to the
wall of the bath, that is the reagent flows
along the walls of the bath.
b. The tip of the sampling probe is aligned
with this delivery port on the bath.
3. Simultaneous delivery of sample and reagent at
a single point on the curved wall of the bath:
a. Sets up a flow that produces a thoroughly
mixed dilution.
b. Sets up a consistent (counterclockwise)
swirling motion that ensures reproducible
sample delivery from the probe.
4. Point out the angled reagent delivery port on
each dilution bath.
5. If you are letting the instrument cycle as you
explain the flow, you may want to have the
trainee focus on sample/reagent delivery into
each bath.
Making the First Dilution
1. The horizontal traverse assembly positions the
sampling probe over the DIL 1/HGB bath.
2. The vertical traverse assembly moves the probe
downward into the bath. The probe tip is
positioned so that a tangential flow can occur as
blood and reagent are delivered to the bath.
3. 10 µL of the whole-blood sample partitioned for
making the first dilution and 1.7 mL of Diluent
are simultaneously dispensed into the
DIL 1/HGB bath.
4. Mixing bubbles enter the bath to make a
uniform suspension of cells.
5. This 1:170 dilution is commonly referred to as
the first dilution.
Tangential flow
Bath
7616002
Probe
Reagent
input
DIL 1
HGB
DIFF RBC BASO
WBC
RINSE
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