PN 177196BB
8-12
SYSTEM DIAGNOSTICS AND MAINTENANCE
RECOGNIZING NORMAL INSTRUMENT OPERATION - CYCLE DESCRIPTION
Making the WBC/BASO Dilution
1. The horizontal traverse assembly positions the
sampling probe over the WBC/BASO bath.
2. The vertical traverse assembly moves the probe
downward into the bath. The tip of the probe is
positioned so that a tangential flow can occur.
3. 10 µL of the whole-blood sample and 2.0 mL of
WBC Lyse are simultaneously dispensed into
the bath, making a final dilution of 1:200.
4. Mixing bubbles enter the bath to make a
uniform suspension of cells.
The WBC Lyse destroys the RBCs and the
specific lytic action on the WBCs differentiates
the basophils from other WBCs.
5. This dilution is used to:
a. Determine the WBC count
b. Develop the WBC/BASO histogram from
which the BASO count is obtained.
Making the DIFF Dilution
1. The horizontal traverse assembly moves the
sampling probe over the DIFF bath.
2. The vertical traverse assembly moves the probe
downward into the bath. The tip of the probe is
positioned so that a tangential flow can occur.
3. 25 µL of the whole-blood sample and 1.0 mL of
Fix reagent are simultaneously dispensed into
the bath.
4. Mixing bubbles enter the bath to make a
uniform suspension of cells.
The Fix reagent lyses the red blood cells,
stabilizes the WBCs in their native form, and
differentially stains the lymphocytes,
monocytes, neutrophils, and eosinophils, with
the eosinophils staining most intensely.
5. After 12 seconds of incubation, the staining
process inside the DIFF bath is stabilized by
adding another 1.0 mL of Diluent which stops
the cytochemical reaction and makes a final
dilution of 1:80.
6. This dilution is used to:
a. Count WBCs for a secondary WBC count.
b. Develop the DiffPlot for detemining the
percent of lymphs, monos, neuts and eos.
DIL 1
HGB
DIFF RBC
BASO
WBC
RINSE
DIL 1
HGB
DIFF RBC BASO
WBC
RINSE
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