PN 177196BB
1-18
GETTING TO KNOW YOUR INSTRUMENT
REAGENTS AND INSTRUMENT WASTE
D Topic Notes and Tasks
Reagents
1. A
C
•T 5diff AL hematology analyzer uses four reagents to dilute and prepare the blood
samples for analysis and one to clean the Analyzer.
a. Four of these reagents are housed in the reagent compartment.
b. Open the reagent compartment door, identify each of the reagents, and review the
information for that reagent listed below.
Note: Service trainers may want to identify and review the function of each reagent
as they install it.
c. The fifth reagent is a 20 L container of Diluent.
1) The Diluent generally sits on the floor below the instrument.
2) Locate the Diluent container and review its function as listed below.
d. To obtain the optimum performance characteristics stated in the Instructions for
Use manual, use the following reagents on your A
C
•T 5diff AL hematology analyzer.
Reagent Label Color Function CBC DIFF
Open Container
Stability
A
C
•T 5diff Diluent
Purple r Isotonic solution that dilutes the sample
r Stabilizes cell membranes
r Conducts aperture current
r Provides the sheath medium for focused
flow through the flow cell
r Rinses Analyzer components after analysis
r Contains less than 0.1% sodium azide which
may cause an explosion if not properly
flushed down the drain with large volumes
of water
ccSame as shelf life
(date printed on
the container)*
A
C
•T 5diff Hgb Lyse
Orange r Disrupts erythrocytes (lysis)
r Frees hemoglobin
r Reduces cellular debris
r Converts hemoglobin to a stable cyanide-
containing pigment
r Contains potassium cyanide, a quarternary
ammonium salt; special instructions are
provided for proper disposal
c 90 Days
A
C
•T 5diff WBC Lyse
Yellow r Dilutes the sample for the total WBC count
r Specifically differentiates between the
basophils and other white blood cells for the
basophil percentage analysis
r Lyses red blood cells
r Reduces cellular debris
ccSame as shelf life
(date printed on
the container)*
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