PN 177196BB
1-19
GETTING TO KNOW YOUR INSTRUMENT
REAGENTS AND INSTRUMENT WASTE
1
2. Always handle the reagents as instructed in the Instructions for Use manual.
a. When a reagent arrives, inspect the containers for any indication the reagent has
been frozen. Discard any reagent that you know or suspect has been frozen.
Note: If a reagent is frozen during transport or storage, active components may
separate (layer) which can cause inconsistency of components and affect parameter
results.
b. Gently mix the reagent containers by inversion before storing them.
Note: Allow sufficent time for microbubbles to dissapate before using the reagents
as microbubbles can increase background counts and affect parameter results.
c. Store all reagents at an ambient temperature of 18°C to 25°C.
3. When changing a reagent:
a. Do not pool reagents; pouring reagents from one container to another may
contaminate the new reagent container which will cause unacceptable background
results, especially for PLT.
b. Make sure the lot number on the new container is the next one in ascending
chronological and container order.
c. Make sure the label on the stopper assembly tubing correlates with the reagent
name on the new container.
d. When transferring the stopper assembly from the old container to the new, do not
touch the pickup tubes or lay the assembly on an uncovered tabletop or the floor.
1) If the pickup tube portion of a stopper assembly is contaminated during
transfer, bacterial and/or fungal growth may occur inside the reagent container.
2) Contamination may cause unacceptable background results especially for PLT.
3) If a pickup tube touches you or anything outside the container, flood the
pickup tube with distilled water then wipe it with a lint-free tissue before
inserting it in the new reagent container.
e. Always dispose of reagents and containers according to your laboratory’s protocol.
4. From the Main Menu screen, click and review the Reagent Status screen.
A
C
•T 5diff Fix
Green r Lyses red blood cells
r Preserves the white blood cells in their
native state
r Stains the granules of eosinophils,
neutrophils, and monocytes
r Provides the dilution used to differentiate the
white blood cell subpopulations using the
absorbance cytochemistry technology
c Same as shelf life
(date printed on
the container)*
A
C
•T 5diff Rinse
Blue r Cleans and rinses the diluter parts
r Prevents protein buildup
r Eliminates routine aperture bleaching
ccSame as shelf life
(date printed on
the container)*
* This open container stability claim applies only if the reagent is properly connected to the Analyzer with an
approved reagent pickup and cap.
Reagent Label Color Function CBC DIFF
Open Container
Stability
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