20 ChroMate™ Operator’s Manual Doc. 4300 Rev. E
4. OPERATING PROCEDURES
Section 4.1 – General Selections provides questions for review before beginning a test,
and information about plate format selection
Section 4.2 - Assay Modes mode-specific description for each general purpose
calculation program
4.1 General Selections
For every test, the instrument will require a mode selection and filter combination.
Thereafter, only those questions that pertain to the mode selected will be asked. In the
absorbance mode, for example, there are no further selections required.
Review the following questions before beginning a test (refer to the reagent kit insert for
required information):
1. What calculating program will be used?
2. What filters are optimal? (operating and differential wavelengths)
3. Is a blank required? Desired?
4. How many calibrators will be used?
NOTE: For the purpose of this manual, the terms calibrator and standard are
used interchangeably to designate reference materials of known
concentrations
5. What is the calibrator value or values?
6. Will calibrators and /or specimens be read in duplicate, or singly?
NOTE: For the purpose of this manual, the terms specimen and sample are
used interchangeably to mean materials of unknown concentrations.
7. Will locations of 1 or more controls be marked? If so, indicate plate number,
row number, and well number for each.
8. Will acceptance cutoffs or ranges for controls be entered for automatic
comparison? If so, what cutoffs or ranges are to be used for each?
9. Will a cutoff value be used to label positive samples? If so, the value which
begins the positive range will be required.
10. Will a cutoff value be used to label negative samples or define an equivocal
zone? If so, results less than what cutoff should be labeled as negative?
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