2 ChroMate™ Operator’s Manual Doc. 4300 Rev. E
User-programmable Memory
ChroMate software allows the operator to create, edit and store assays.
Standard curves are also stored in this memory. Test protocols remain
stored until either changed or deleted by the user.
1.1.3 Principles of Operation
The plate carrier precisely positions each well into the optical path for reading.
Light energy from a lamp is focused by an integral lens, directed through an
aperture, and then passed vertically through the sample. A wheel below the
sample positions the filters so that readings can be taken very quickly at both the
operating and differential wavelengths. (Using bichromatic differential
absorbance values corrects for optical imperfections in the plastic wells and
removes the effects of meniscus and turbidity.) A photo detector converts
transmitted light energy into electrical signals, which are amplified and
interpreted.
The ChroMate™ 4300 Microplate Reader’s optical system provides an
economical and low maintenance design which reads 8 wells simultaneously. A
96 well plate can be read and printed in the absorbance mode in approximately
eight seconds.
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