ImageQuant LAS 4000 setting
Reagent nameClassification
IrisFilterLightMethod
Alexa 680 0.85R670Red (630nm
Epi)
Fluorescence:Cy5
2.8L41UV (365nm Epi)Fluorescence:DAPIQ-dot
0.85IR785Infrared
(710nm Epi)
Fluorescence:IRDy781
0.85IR785Infrared
(710nm Epi)
Fluorescence:IRAlexa 680
0.85IR785Infrared
(710nm Epi)
Fluorescence:IRAlexa 700
0.85Y515-DiBlue (460nm
Epi)
Fluorescence:SYBR GreenAttophos
1
Fluorescence dye (Chemi-
fluorescence)
0.85Y515-DiBlue (460nm
Epi)
Fluorescence:SYBR GreenECL Plus
2.8ThroughWhite (Trans)Digitization:TransSilver stain
Digitization
2.8ThroughWhite (Trans)Digitization:TransCBB stain
2.8ThroughWhite (Trans)Digitization:TransX-ray film
2.8ThroughWhite (Epi)Digitization:EpiNBT/BCIP
1
Attophos cannot be used for detecting nucleic acid on a nylon membrane.
Note:
With regard to patents owned by third parties related to, among other things, sample
preparation, we recommend that you consult with a lawyer or patent attorney about
obtaining a license from the third parties.
A.4 Main specifications
3 200 000 pixels (Fujifilm super CCD)CCD
-25°CCooling temperature
16 bitRecorded image:
8 bitFocusing:
Number of gradations
1/100 seconds to 2 hoursExposure time
(Images can be continuously exposed for up to 30 hours using ImageQuant LAS 4000
Control Software.)
ImageQuant LAS 4000 User Manual 28-9607-42 AB 127
A Appendix
A.3 Detection Reagents and corresponding settings