4.7 Exposing fluorescence (Epi illumination)
This section describes how to expose fluorescent samples using Epi illumination.
Ensure that the correct Epi lights and filter are installed. See Chapter 3 Exchanging accessory parts, on
page 17.
Place a sample on the Epi tray. Put the tray into the ImageQuant LAS 4000 and close the door.
Note:
•
Make sure that sample is suitably placed on the tray for the desired tray position.
•
Ensure that a suitable method exists and that the ImageQuant LAS 4000 is properly
calibrated. See Section 4.16 Creating a new method and performing flat frame
calibration, on page 78.
ImageQuant LAS 4000 User Manual 28-9607-42 AB 47
4 Operating the ImageQuant LAS 4000
4.7 Exposing fluorescence (Epi illumination)