Calibration and controls
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In order not to contaminate the calibrator or control with cleaning solution, be
sure to dry the aspiration pipette manually on the outside at each control blood
run. Not following this discipline might give dropping values over time on the
concentration parameters like RBC, HGB, WBC and PLT. See picture below.
.
10.3 Calibration on a Known Sample
The following procedure can be used in case no Hematology Blood Calibrator is
available or if the recommended transport conditions of such cannot be met.
The RBC, WBC and PLT values can be obtained by using a reference analyzer or
a microscope method.
The hematocrit should be checked with a micro centrifuge method and the he-
moglobin with the cyan meta-hemoglobin method on a reference photometer
system.
Calculate the MCV by: MCV =HCT/RBC.
Procedure:
1. Make at least 3 HCT determinations with a micro centrifuge and calculate
the mean value.
2. Make at least 3 HGB determinations with the cyan meta-hemoglobin meth-
od and calculate the mean value.
3. Perform blank counts on the instrument and check that the background is
according to the limits stated in chapter Analysing the Sample (Open
Tube) on page 54.
4. Aspirate and analyze the known sample at least six times. Print the parame-
ter results of each count.
Warning
Contamination hazard if
contaminated blood enters
into open cut.
Always wear protective
gloves when handling
blood samples
Important
Dry the aspiration pipette
manually on the outside at
each control blood run
and do not push the con-
trol or calibrator tube
against the upper washing
device. See pictures.
Not following this disci-
pline might give dropping
values for the RBC, HGB,
PLT and WBC parameters.
1132.bmp
1133.bmp
1134.bmp
Dry the aspiration pipette manually
Correct sample handling
1136.bmp
Do not push the sample tube against the
washing device
1135.bmp
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