Waters 2690 - Preparing a New or Dry Separations Module (Sparge-Based)

50 Recommended Preparation Procedures
Figure 6-1 outlines how to prime, equilibrate, and purge a sparge-based Separations
Module that is new or dry.
Note:
All flow chart references refer to sections in the Waters 2690 Separations Module
Operator’s Guide.
Figure 6-1 Preparing a New or Dry Separations Module for Operation (Sparge-Based Unit)
Fill the Solvent
Reservoirs with
Fresh Solvent
Filtered and
Degassed by
Vacuum Filtration
(See Section 2.6.3)
Sparge all
Reservoirs at an
Initial Sparge Rate
of
100%
for at
Least 15 min. Set
an Idle Sparge Rate
of
30
to
50%
(See Section 4.4.4)
Equilibrate for a
Minimum of 10
Column Volumes
(See Section 4.4.5)
Purge the Injector
for a Minimum of 6
Loop Volumes
(See “Purging the
Injector” in
Section 4.4.2)
Perform a Dry
Prime (to Fill Each
Solvent Line) See
Section 4.4.1
Perform a Wet
Prime (for One of
the Solvents) for
5
min at
5.00
mL/min
(See Section 4.4.2)
Repeat the Wet
Prime (at the Same
Rate and Time) for
Each Solvent
To Make a Gradient
Run (for Example,
A:B), Set the A and
B Composition
Fields in the Status
Screen to
50%
,
then Wet Prime for
2
min at
5.00
mL/min
(See Section 4.4.2)
Start