Waters 2690 - Preparing a New or Dry Separations Module (Degasser-Based)

Recommended Preparation Procedures 51
Figure 6-2 outlines how to prime, equilibrate, and purge a vacuum degasser-based
Separations Module that is new or dry.
Note:
All flow chart references refer to sections in the Waters 2690 Separations Module
Operator’s Guide.
Figure 6-2 Preparing a New or Dry Separations Module for Operation (Degasser-Based Unit)
Fill all Four
a
Solvent
Reservoirs with
Fresh Solvent
Filtered and
Degassed by
Vacuum Filtration
(See Section 2.6.2)
Set the Degasser to
Continuous
(See
“Equilibrating
Solvents in the
Vacuum Degasser”
in Section 4.4.1)
Equilibrate for a
Minimum of 10
Column Volumes
(See Section 4.4.5)
Purge the Injector
for a Minimum of 6
Loop Volumes
(See “Purging the
Injector” in
Section 4.4.2)
Perform a Dry Prime
(to Fill Each Solvent
Line and
Degasser Chamber)
(See Section 4.4.1)
Perform a Wet
Prime (for One of
the Solvents) for
10
min at
5.00
mL/min
(See Section 4.4.2)
Repeat the Wet
Prime (at the Same
Rate and Time) for
Each Solvent
To Make a Gradient
Run (for Example,
A:B), Set the A and
B Composition
Fields in the Status
Screen to
50%
,
Then Wet Prime for
2
min. at
5.00
mL/min.
(See Section 4.4.2)
Start
Equilibrate
b
the
Solvent in the
Degasser Chamber
by Setting a Flow
Rate of
0.000
mL/min for
5
min.
(See “Equilibrating
Solvents in the
Vacuum Degasser”
in Section 4.4.1)
Set the Degasser to
Normal
(See “Equilibrating
Solvents in the
Vacuum Degasser”
in Section 4.4.1)
a
To maximize degasser efficiency (and fill all four degasser chambers with solvent), Waters recommends that
all four solvent reservoirs be filled (even if four solvents are not used). If you choose water as one of the
“unused” solvents, change the water weekly to prevent bacterial contamination.
b
Except for the short period of time required to equilibrate solvents in the degasser, do not run the degasser
without solvent flow. Refer to the “Degasser Considerations” discussion, Section 1.5, for more details.