YSI 2900 - 8.2.3 Galactose

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8.2.3 Galactose
This is a direct reading of galactose in solution at the enzyme sensor. The enzyme Galactose oxidase is immobilized in
the enzyme membrane.
Galactose + O
2 Gal Oxidase
H
2
O
2
+ Galactose Dialdehyde Derivative
System Buffer
YSI 2705
Calibrator Std
n/a
4
Linearity Std
n/a
Membrane
YSI 2702
Membrane
Color
White
Detection
Range
~0.1–25 g/L
Calibration
Point
~2 g/L
(recommended)
Sample Size
25 µL
End Point
30 sec
Typical Working
Life
~10 days
Note: See Appendix B – Concentration
Unit Conversion if concentration unit
conversion is required.
Special Considerations:
• If sample dilution is required, use reagent water.
• The sample should be lactose-free. Lactose, glycerol,
and other galactosides such as raffinose and stachyose
are substrates for galactose oxidase. They may interfere
by producing artificially high galactose readings.
• The enzyme membrane integrity test involving the use of
YSI 2363 Potassium Ferrocyanide solution is not
informative since ferrocyanide exists in the 2705 system
buffer.
• YSI believes that you will be able to measure this analyte
for many applications in the range specified. However,
YSI makes no claims with respect to precision or
linearity. User will need to prepare the calibrator and
linearity standards for your application as YSI does not
currently offer galactose standard solutions.
4
YSI does not currently offer galactose calibrator standard solutions.