YSI 2900 - 8.2.13 Xylose

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8.2.13 Xylose
This is a direct reading of xylose in solution at the enzyme sensor. The enzyme pyranose oxidase is immobilized in the
enzyme membrane.
D-Xylose + O
2 Pyranose Oxidase
H
2
O
2
+ D-xylosone
System Buffer
YSI 2357
Calibrator Std
YSI 2767
Linearity Std
YSI 2768
Membrane
YSI 2761
Membrane
Color
Orange
Detection
Range
0.5–30.0 g/L
Calibration
Point
20.0 g/L
Linearity Check
Point
30.0 g/L
Sample Size
13 µL
End Point
45 sec
Precision
(CV,n=10)
2% or 0.5 g/L,
whichever is
greater
Linearity
±10%
(0.5 to Range Max)
Typical Working
Life
10 days
Note: See Appendix B – Concentration
Unit Conversion if concentration unit
conversion is required.
Special Considerations:
• If sample dilution is required, use reagent water or YSI
buffer.
• The sample must be glucose-free, or at least contain
levels low enough not to interfere with the xylose
reading, since glucose will react with pyranose oxidase.
• See 8.2.17 Simultaneous Glucose and Xylose for xylose
specifications when measuring glucose and xylose
simultaneously.
Response to Related Sugars:
The relative magnitude of interference of related sugars is listed
below. Note that relative responses may vary with both sample
matrix and membrane lot.
Pyranose Oxidase substrates (relative response, based on equal
concentrations):
Xylose = 1.0
Glucose = 0.8
Galactose = 0.6
Cellobiose = 0.02
Mannose = < 0.002
Arabinose = 0.01
Fructose = < 0.002