YSI 2900 - 8.2.12 Sucrose

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8.2.12 Sucrose
This is a direct reading of Sucrose in solution at the enzyme sensor. Three enzymes are co-immobilized in the YSI
Sucrose Membrane: Invertase, Mutarotase, and Glucose Oxidase. Through this chain of reactions the moles of hydrogen
peroxide liberated is directly proportional to the moles of sucrose.
Sucrose + H
2
O
Invertase
α-D-Glucose + (Fructose)
α-D-Glucose
Mutarotase
β-D-Glucose
β-D-Glucose + O
2 Glu Oxidase
H
2
O
2
+ D-Glucono-δ-Lactone
System Buffer
YSI 2357
Calibrator Std
YSI 2780
Linearity Std
YSI 2778
Membrane
YSI 2703
Membrane
Color
Blue
Detection
Range
0.1–25.0 g/L
Calibration
Point
5.0 g/L
Linearity Check
Point
25.0 g/L
Sample Size
25 µL
End Point
30 sec
Precision
(CV,n=10)
2% or 0.02 g/L,
whichever is
greater
Linearity
±2%
(0.1 to Cal Point)
±5%
(Cal Point to Range
Max)
Typical Working
Life
10 days
Note: See Appendix B – Concentration
Unit Conversion if concentration unit
conversion is required.
Special Considerations:
• If sample dilution is required, use reagent water or YSI
buffer.
• The sample must be glucose-free, or at least contain
levels low enough not to interfere with the sucrose
reading. Since the sucrose membrane contains glucose
oxidase, glucose will produce a probe signal.
• See 8.2.16 Simultaneous Glucose and Sucrose for
sucrose specifications when measuring glucose and
sucrose simultaneously.