YSI 2900 - 8.2.17 Simultaneous Glucose and Xylose

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8.2.17 Simultaneous Glucose and Xylose
Refer to the previous sections on Glucose and Xylose for theoretical and special considerations, then follow the
instructions below to set up your instrument for simultaneous determination.
A Probe
B Probe
Chemistry
Glucose
Xylose
System
Buffer
YSI 2357
Calibrator
Std
YSI 2776
YSI 2767
Linearity Std
YSI 1531
(or 2356)
YSI 2768
Membrane
YSI 2365
YSI 2761
Sample Size
13 µL
Unit of
Conc.
g/L
Cal Value
2.50
20.0
End Point
45 sec
Note: See Appendix B – Concentration
Unit Conversion if concentration unit
conversion is required.
Since the xylose membrane also responds to glucose, the xylose
measurement is based on an algorithm that simultaneously
measures both xylose and glucose. The algorithm utilized takes
into account the responsiveness of the xylose membrane to
glucose and the slight response of the glucose membrane to
xylose.
Special Considerations:
• When measuring glucose and xylose, the glucose and
xylose sensors must be located in the same module.
• As with most analytical methods there is a concentration
range that provides optimal accuracy and precision. The
YSI xylose and glucose methods are specified
independently for performance; however these two
analytes frequently occur together.
Glucose and Xylose Specifications:
When simultaneously measuring glucose and xylose, the glucose
and xylose precision and linearity are as follows:
Precision (CV,n=10): 2% or 0.1 g/L, whichever is greater
Linearity: ±5% or 0.5 g/L, whichever is greater