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4.7.1 Prepare Buffer
4.7.1.1 From powder concentrate:
1. Place about 500 mL of reagent water (distilled or deionized) into a 1000 mL flask or other clean container.
2. Add two packages of powder buffer concentrate and stir.
3. Add more reagent water until the total volume of solution is between 900 and 1000 mL.
4. Stir as necessary until the buffer chemicals have completely dissolved.
4.7.1.2 From liquid concentrate:
Mix the content of the bottle of liquid buffer concentrate with enough reagent water (distilled or deionized) to make
1000 mL.
4.7.2 Install Buffer Solution(s)
Unscrew and remove the lid from one of the buffer bottles.
IMPORTANT: When adding fresh buffer to the Buffer Supply Bottles, make every effort to avoid contamination of
the lid and level sensor assemblies.
Pour the prepared buffer into the buffer bottle.
Install the bottle in the rack as indicated on the display.
Replace the bottle lid.
Install Calibrator Solution(s) 4.8
Caution: To prevent possible damage due to an electrostatic discharge, do NOT touch the metal
tips of the connectors located at the ends of the bottle leads. Handle only the insulated section of
the connectors.
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