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Abbott AEROSET - Acid Phosphatase; Method Comparison; Assay-Specific Information

Abbott AEROSET
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PROPRIETARY INFORMATION
CONFIDENTIAL MATERIAL
Not for Customer Distribution
AEROSET
®
System Troubleshooting Guide 101
94816-107—November 2004
Assay-specific Information
Acid Phosphatase
Method Comparison
The Abbott Clinical Chemistry Acid Phosphatase (ACP) assay
compares well to some other assays and not as well to others.
Review of CAP proficiency testing survey results indicates the
degree of comparability that can be expected. For two survey
specimens, the mean values are listed in the following table.
Array and AEROSET values for ACP agree with each other, as
do the Vitros and Paramax, but the Array/AEROSET do not
agree with Vitros/Paramax. ACP values for three other CAP
specimens tested by Paramax and AEROSET demonstrate
similar disparity (1.93, 10.85, and 7.73 U/L by Paramax; 5.67,
40.39, 29.19 U/L by AEROSET).
Method comparison data for AEROSET and Paramax yielded
the following least squares equation:
AEROSET = 3.61 (Paramax) - 2.96, r = 0.992, N = 78
When the same data were analyzed using Passing-Bablok, the
equation was:
AEROSET = 2.86 (Paramax) - 1.59, r = 0.992, N = 78
Instrument Specimen 1 (U/L) Specimen 2 (U/L)
Array 1.55 19.78
AEROSET 1.66 21.70
Paramax 0.44 5.57
Vitros 0.65 5.55

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