PROPRIETARY INFORMATION
CONFIDENTIAL MATERIAL
Not for Customer Distribution Assays/Reagents
AEROSET
®
System Troubleshooting Guide 81
94816-107—November 2004
Sample Blanking
Self Blank vs. Non-Self Blank
Self Blank
The blank is done in the same cuvette as assay measurement. It
is typically done with R1 and sample immediately before R2 is
added. On the Base page of the Assay Configuration screen,
in the Sample Blank Test field, you must select the same assay
name as the assay you are configuring, then a Blank Read Time
must be defined. The absorbance value measured during the
Blank Read Time is corrected for the sample/reagent dilution
ratio, then is subtracted from the absorbance value measured
during the Main Read Time. If the Blank Read Time is defined
after the addition of R2, the absorbance value is not corrected
for the dilution ratio.
Non-Self Blank
A separate assay parameter file is defined to measure the blank
reading. All parameters must be defined as if it was a
“stand-alone” assay. This stand-alone blank assay should have
nothing selected in the Sample Blank Test field or in the Blank
Read Time field. For example, for the blank test, you could use
saline or water, and sample. On the Base page of the Assay
Configuration screen of the assay to be blanked, you must
select the assay name of the configured blank assay in the
Sample Blank Test field. The Blank Read Time fields must
remain zero. When the assay is ordered, the AEROSET System
dispenses the sample and reagent defined in the assay
configuration into one cuvette and dispenses the sample and
reagent defined in the blank configuration into another
cuvette. They are treated as two separate tests to be dispensed
and measured. When measurement is completed, the system
subtracts the absorbance value measured during the Main
Read Time of the blank assay from the absorbance value
measured during the Main Read Time of the assay.
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