PROPRIETARY INFORMATION
CONFIDENTIAL MATERIAL
Assays/Reagents Not for Customer Distribution
42
AEROSET
®
System Troubleshooting Guide
94816-107—November 2004
Chemical Reactions for Clinical Chemistry Assays
Assay
Name
Reaction Tips
ACP
α-Naphthylphosphate α-Napthol + PO
4
-3
α-Napthol + Diazotized 2-amino-5-chlorotoluene Dye complex
Use fresh,
nonhemolyzed,
nonicteric, and
nonlipemic serum
samples. All
specimens need to be
stabilized with 50 µL
Liquid Stabilizer for
every 1 mL of serum.
The acidified serum
should be analyzed
immediately, or
stored at 2 to 8°C, or
frozen. Calibration is
stable for
approximately five
days and performed
using a water blank.
Sample Interference
Indices may assist
with determination of
sample integrity.
AlbG
(BCG)
Albumin + BCG Colored complex
BCG = Bromcresol green
Results are running
high, due to
non-specific protein
binding. Recommend
AlbP if more
specificity needed.
AlbP
(BCP)
Human Albumin + BCP Colored complex
BCP = Bromcresol purple
Human albumin
specific.
AlkP
p-Nitrophenylphosphate + H
2
O p-Nitrophenol + phosphate
AlkP reagent contains
AMP buffer.
ALT
L-Alanine + α-Ketoglutarate Pyruvate + L-glutamate
Pyruvate + NADH L-lactate + NAD
+
LD = Lactate dehydrogenase
NADH = Nicotinamide adenine dinucleotide
Do not use plasma
samples collected
using ammonium
heparin.
* MULTIGENT
®
assay
ACP
AlkP
Mg
+2
, Zn
+2
ALT
LD
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