PROPRIETARY INFORMATION
CONFIDENTIAL MATERIAL
Not for Customer Distribution Assays/Reagents
AEROSET
®
System Troubleshooting Guide 43
94816-107—November 2004
ALT-A
L-Alanine + α-Ketoglutarate Pyruvate + L-glutamate
Pyruvate + NADH L-lactate + NAD
+
LD = Lactate dehydrogenase
NADH = Nicotinamide adenine dinucleotide
Activated reagent
contains pyridoxal-5-
phosphate (P-5-P).
Do not use plasma
samples collected
using ammonium
heparin.
AmpM
Antibody to AmpM (R1) + G-6-PD-AmpM (R2) (Active)
G-6-PD-AmpM:Antibody complex (Inactive)
G-6-PD-AmpM:Antibody complex (Inactive) + AmpM
G-6-PD-AmpM (Active) + AmpM:Antibody complex
G-6-PD-AmpM (Active) + NAD + Glucose-6-phosphate
NADH + 6-phosphogluconate
G-6-PD = Glucose-6-phosphate dehydrogenase from bacterial source
NADH = Nicotinamide adenine dinucleotide
Use Multi DOA Cutoff
calibrator in duplicate
for calibration. Cutoff
calibrator value is
zero on
CALIBRATOR/
CONTROL screen.
Amy
10 CNPG
3
9 CNP + 1 CNPG
2
CNPG
3
= 2-chloro-4 nitrophenyl-α D-maltotrioside
Chromophore is 2-
chloro-4-nitrophenol
(CNP), read at
404 nm.
ApoA
Apo-A1 + PEG/buffer (R1) + Antibody to Apo-A1 (R2) Turbidity due
to Apo-A1:Antibody complex
PEG = Polyethylene glycol
ApoB
Apo-B + PEG/buffer (R1) + Antibody to Apo-B (R2) Turbidity due
to Apo-B:Antibody complex
PEG = Polyethylene glycol
AST
L-Aspartate + α-Ketoglutarate Oxaloacetate + L-glutamate
Oxaloacetate + NADH L-Malate + NAD
+
MD = Malate dehydrogenase
NADH = Nicotinamide adenine dinucleotide
Do not use plasma
samples collected
using ammonium
heparin.
Assay
Name
Reaction Tips
* MULTIGENT
®
assay
ALT, P-5-P
LD
α-amylase
AST
MD
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