Abbott AEROSET - Page 52

PROPRIETARY INFORMATION
CONFIDENTIAL MATERIAL
Assays/Reagents Not for Customer Distribution
52
AEROSET
®
System Troubleshooting Guide
94816-107—November 2004
LDL*
HDL, LDL, VLDL, Chylomicrons LDL (non-LDL particles
consumed in a non-color
forming reaction)
LDL Cholesterol Cholesterone + H
2
O
2
H
2
O
2
+ DSBmT + 4-AAP Quinoneimine dye + H
2
O
DSBmT = N,N-bis(4-sulphobutyl)-m-toluidine-disodium
4-AAP = 4-Aminoantipyrine
MULTIGENT Direct
LDL must not be on
the same line as the
Lipase assay.
Samples may be
frozen one time at
-80°C.
Lip
1,2 Diglyceride + H
2
O 2-Monoglyceride + fatty acid
2-Monoglyceride + H
2
O Glycerol + fatty acid
Glycerol + ATP Glycerol-3-phosphate + ADP
Glycerol-3-phosphate + O
2
Dihydroxyacetone phosphate + H
2
O
2
2H
2
O
2
+ 4-AAP + TOOS Quinoneimine dye + 4H
2
O
4-AAP = 4-Aminoantipyrine
TOOS = N-ethyl-N-(2-hydroxy)-3-sulfopropyl-m-toluidine
There are reports of
interference by
non-Abbott HDL
methods. Correct
SmartWash™
configuration is
critical for some
assays.
Serum only.
Must not be on the
same line as
Triglyceride, Calcium,
Urine Calcium,
Cholesterol, CO
2
,
MULTIGENT LDL,
and Uric Acid.
Meth
Antibody to Methadone (R1) + G-6-PD-Methadone (R2) (Active)
G-6-PD-Methadone:Antibody complex (Inactive)
G-6-PD-Methadone:Antibody complex (Inactive) + Methadone
G-6-PD-Methadone (Active) + Methadone:Antibody complex
G-6-PD-Methadone (Active) + NAD + Glucose-6-phosphate
NADH + 6-phosphogluconate
G-6-PD = Glucose-6-phosphate dehydrogenase from bacterial source
NADH = Nicotinamide adenine dinucleotide
Use Multi DOA Cutoff
calibrator in duplicate
for calibration. Cutoff
calibrator value is
zero on
CALIBRATOR/
CONTROL screen.
Assay
Name
Reaction Tips
* MULTIGENT
®
assay
Detergent 1
CO
CE
POD
Detergent 2
Peroxidase
Lipase
MGLP
GK
GPO
POD