Automatic Liquid Sampler and Injector
Preparing the solvent and waste bottles
Released: March 2004 6850 Series II GC User Information page 220 of 256
Figure 38. Vial cap aperture specifications
Filling sample vials
Recommended fill volumes are:
• 1 mL for the 2-mL vial
• 50 µL for the 100-µL vial
Refer to the Sampling Techniques Handbook on the CD-ROM for more infor-
mation on sample vial volumes and how it can effect chromatographic results.
Preparing the solvent and waste bottles
Solvent bottles hold solvent for rinsing the syringe between injections. The
liquid sampler or injector discards the solvent washes and sample washes into
waste bottles. The number of samples that can be analyzed may be limited by
the wash or waste bottle capacity shown in Figure 39 and Figure 40.
Rinse and fill each solvent bottle with 4 to 4.5 mL of fresh solvent. The liquid
level will be near the shoulder of the bottle. Good laboratory practice dictates
using no more than 2.0 mL of the 4.5 mL solvent for syringe washes. The needle
tip draws solvent at a height of 18.5 mm from the bottom of the vial. See
Figure 39.
Figure 39. Position of syringe tip when withdrawing solvent
5.58 mm (0.220 in.) recommended
4.65 mm (0.183 in.) minimum
500 washes of a 5 µL syringe
18.5 mm
4.5 mL fill volume
2.5 mL solvent remains
2.0 mL usable solvent volume
250 washes of a 10 µL syringe
100 washes of a 25 µL syringe
50 washes of a 50 µL syringe
25 washes of a 100 µL syringe
MINIMUM
SOLVENT
LEVEL
Capacity of one solvent bottle
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