Automatic Liquid Sampler and Injector
Cool on-column injection
Released: March 2004 6850 Series II GC User Information page 228 of 256
Cool on-column injection
Technique
If using a cool on-column inlet, the automatic liquid sampler or injector
performs injections directly onto 250-µm, 320-µm, and 530-µm columns.
When performing on-column injections, the injector:
• Slows the carriage speed so the overall injection time increases to
500 milliseconds.
• Lowers the tip of the syringe needle an additional 19 mm onto the column.
Adapting for cool on-column injection
To adapt the injector and GC for cool on-column use:
1. Select the on-column syringe needed for the column size. See your Agilent
catalog for consumables and supplies and “Maintaining a cool on-column
inlet” on page 166 for a list of parts.
2. Prepare the GC inlet. See “Maintaining a cool on-column inlet” on
page 166.
• Check your needle to column size.
• Verify that the insert matches the needle size.
• If necessary, replace the septum.
3. Verify that the correct needle support assembly is installed in the injector.
Replace it if necessary. See “Replacing the needle support assembly” on
page 224.
4. If necessary, reinstall the injector onto the GC.
5. Install the syringe. See “Installing a syringe” on page 222.
6. Rotate the turret clockwise until it stops, then verify the installation by
manually sliding the syringe carriage down until the needle enters the
inlet.