Agilent 1260 Infinity RID User Manual 121
Test Functions
8
Refractive Index Calibration
5 Purging the sample and reference cells.
a The purge valve will automatically switch to the ON position.
b Using a syringe or LC pump flush the sample and reference cell with
about 20 mL of LC grade water. (If an Agilent pump is part of the system,
the pump section is active).
c The purge valve will automatically switch to the OFF position when you
click continue.
6 Fill the sample cell with calibration solution.
a Remove the inlet capillary or flushing syringe from the in port.
b Take the syringe and fix the needle to the syringe adapter.
c Suck about 1.5 mL of the calibration sample into the syringe.
d Keep the syringe in a horizontal position.
e Remove the needle.
f Add the filter to the syringe and fit the needle to filter.
Figure 33 Syringe with Sample Filter
g Lift the needle tip and carefully eject approximately 0.5 mL to remove air
out of the syringe and to flush the needle.
h Add the PEEK fitting to the needle tip and fix both at the flow cell inlet.
i Slowly inject about 1.0 ml and wait for about 10 s to inject another
0.1 mL. This will assure that the cell is filled properly.
HVbeaZ[^aiZg
NOTE
Do not inject the calibration solution without the sample filter.
To Next Page
Loading...
Need help?
General
