Agilent 1260 Infinity RID User Manual 75
Using the Refractive Index Detector
4
Running a Checkout Sample
The resulting chromatogram is shown in the figure below:
Figure 27 Isocratic Standard Sample Chromatogram
NOTE
The resulting chromatogram should only be seen as a qualitative example, the checkout
procedure is not meant as a quantitative procedure. Its intent is only to verify the presence
of the four peaks from the checkout sample - nothing more.
Please be aware of the large negative air / solvent peak from the injection (cut out from the
bottom of the following figure) prior to the first peak of interest. This is to be expected in a
regular chromatogram, especially if a non-degassed sample is injected into degassed
solvent and the sample solvent properties don’t perfectly match with the mobile phase
properties. Only similar zooming factors in the display of a chromatogram will lead to
similar looking results.
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