Methods and Users 4-7
Procedure
To create a method:
Step Action
1 From the Main Menu, press F2 (Create).
The Create/Edit screen appears.
2 Change times or temperatures or number of cycles by highlighting each parameter
you want to change, typing in a new value with the numeric keys, then pressing
Enter.
If the method you want to create is more complex that the one shown above,
continue with the procedure and create and store a method as close to your ideal
one as possible, then see “Changing a Method Using Advanced Features” on
page 4-9.
3 When you have finished changing parameters, press F2 (Store).
The Store Method on Instrument screen appears.
1 Hld 3 Tmp 25 Cycles 2 Holds
94.0 94.0
5:00 0:30
55.0
0:30
72.0
0:30
72.0
7:00
4.0
F1 F3 F4 F5F2
Start CancelMorePrintStore
Parameter Explanation
Temperature The number above the graph in °C. Valid range is 4.0 °C to
99.9 °C.
Time The number below the graph in min:sec format. Valid range is
00.00 to 99:59; however, a value of 99:00 or greater creates a
hold time of ∞ that lasts indefinitely. A hold time of ∞ can be used
only as the final hold time on the graph (in the post-PCR portion).
For more information, see “About Post-PCR Parameters” on
page 4-8.
Hold Called Hld, Tmp, or Holds in the top line of the screen. This
parameter determines the number of time/temperature segments
in each portion of the graph delineated by dashed lines. In the
pre-PCR portion, this value is usually 1. In the PCR portion
(middle), 3 is typical for many PCR amplifications: template
denaturation, primer annealing, and primer extension, although
the valid range is 2–6.
Cycles The number of times you want the PCR portion (middle) of the
graph to repeat. Valid range is 2–99.
Store Method on Instrument
F1 F3 F4 F5F2
Accept CancelMethod
User
Method:exp000
Free Mem: 122 methods 946 segments
User:
adrian
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