Applied Biosystems 2720 User Manual

4-10 Methods and Users
Inserting a Hold
A hold is a single time/temperature segment of a method, e.g., samples are held at
94.0 °C for 5 minutes, 00 seconds (5:00).
There are two ways you can insert a hold into a method:
♦ You can increment the value of the Hld, Tmp, or Holds fields on the Create/Edit
screen, then press the Enter key. This adds a hold at the far right of the pre-PCR,
PCR, or post-PCR segment, separated by dashed lines, respectively.
♦ You can use the procedure described below to insert a hold to the left of any
segment.
To insert a hold:
Step Action
1 From the Create/Edit screen, use the arrow keys to highlight a time or temperature
parameter to the left of which you want to insert a hold.
2 Press F4 (More).
The Insert-Delete-More screen appears.
Note The Modify function (F1) also displays if the highlighted parameter was in
the PCR segment.
3 Press F2 (Insert).
The Insert screen appears.
Note The Pause function will not display on the Insert screen if the highlighted
segment already has a pre-programmed pause or if highlighter is not on a PCR
segment parameter.
1 Hld 3 Tmp 25 Cycles 2 Holds
94.0
94.0
5:00 0:30
55.0
0:30
72.0
0:30
72.0
7:00
4.0
F1 F3 F4 F5F2
MoreDeleteInsert
1 Hld 3 Tmp 25 Cycles 2 Holds
94.0
94.0
5:00 0:30
55.0
0:30
72.0
0:30
72.0
7:00
4.0
F1 F3 F4 F5F2
Hold CancelPauseCycle

Applied Biosystems 2720 Specifications

General

Brand	Applied Biosystems
Model	2720
Category	Laboratory Equipment
Language	English