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Applied Biosystems 7500 User Manual

Applied Biosystems 7500
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Appendix A
Moving the 7300/7500/7500 Fast System
116 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
5. Run a TaqMan
®
RNase P Instrument Verification
plate (see page 87).
If the run passes, recalibrations are not
necessary.
If the run fails, perform steps steps 6
through 9 to recalibrate the instrument.
6. Perform an ROI calibration (see page 35).
7. Perform a Background calibration (see page 49).
Perform an optical calibration if you are using a
7500 or 7500 Fast system (see page 59).
8. Perform a pure dye calibration (see page 67).
9. Perform an instrument verification run (see
page 87).

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Applied Biosystems 7500 Specifications

General IconGeneral
Detection MethodFluorescence
Sample Capacity96-well plate
Thermal CyclerYes
Temperature Uniformity±0.5°C
TypeReal-Time PCR System
Thermal Accuracy±0.25°C
Detection Channels5
Weight45 kg
Power Requirements100-240 V, 50/60 Hz
Dynamic Range9 logs
Reaction Volume Range10-100 µL
Temperature Range4°C to 100°C
Thermal Cycler Block96-well
SoftwareSDS Software
DetectionMultiplex (up to 5 colors)

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