Applied Biosystems 7500 User Manual

Appendix A
Moving the 7300/7500/7500 Fast System
116 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
5. Run a TaqMan
®
RNase P Instrument Verification
plate (see page 87).
• If the run passes, recalibrations are not
necessary.
• If the run fails, perform steps steps 6
through 9 to recalibrate the instrument.
6. Perform an ROI calibration (see page 35).
7. Perform a Background calibration (see page 49).
Perform an optical calibration if you are using a
7500 or 7500 Fast system (see page 59).
8. Perform a pure dye calibration (see page 67).
9. Perform an instrument verification run (see
page 87).

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)