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Applied Biosystems 7500 User Manual

Applied Biosystems 7500
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viii Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Chapter 3 Installing the Software and Powering On the Instrument 23
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Installing the SDS Software for the 7300/7500/7500 Fast System . . . . . . . . . . . . . . 25
Powering On the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Performing the Instrument Function Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Chapter 4 Performing the
Regions of Interest (ROI) Calibration 35
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Preparing the ROI Calibration Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Creating a Plate Document for the ROI Calibration . . . . . . . . . . . . . . . . . . . . . . . . . 41
Performing the ROI Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Chapter 5 Performing the
Background Calibration and Optical Calibration 49
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Preparing the Background Calibration Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Creating a Plate Document for the Background Calibration . . . . . . . . . . . . . . . . . . . 54
Performing the Background Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Analyzing the Background Calibration Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Performing the Optical Calibration for the 7500/7500 Fast System . . . . . . . . . . . . . 59
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Chapter 6 Performing the Pure Dye Calibration 67
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Preparing for the Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
Creating a Plate Document for the Pure Dye Calibration . . . . . . . . . . . . . . . . . . . . . 72
Preparing Plates and Performing the Pure Dye Calibration . . . . . . . . . . . . . . . . . . . 73
Analyzing the Pure Dye Calibration Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Evaluating Pure Dye Calibration Spectra . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
Chapter 7 Verifying the Instrument Performance 87
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
Preparing the TaqMan
®
RNase P Verification Plate . . . . . . . . . . . . . . . . . . . . . . . . . 90
Creating a Plate Document for the RNase P Verification Run . . . . . . . . . . . . . . . . . 92
Performing the Verification Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
Analyzing the TaqMan
®
RNase P Plate Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99

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Applied Biosystems 7500 Specifications

General IconGeneral
Detection MethodFluorescence
Sample Capacity96-well plate
Thermal CyclerYes
Temperature Uniformity±0.5°C
TypeReal-Time PCR System
Thermal Accuracy±0.25°C
Detection Channels5
Weight45 kg
Power Requirements100-240 V, 50/60 Hz
Dynamic Range9 logs
Reaction Volume Range10-100 µL
Temperature Range4°C to 100°C
Thermal Cycler Block96-well
SoftwareSDS Software
DetectionMultiplex (up to 5 colors)

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