Applied Biosystems 7500 User Manual

Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide ix
Chapter 8 Powering Off the
7300/7500/7500 Fast System 101
Performing a Short-Term Shutdown . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102
Performing a Long-Term Shutdown . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
Appendix A Maintaining the Instrument 105
Recommended Maintenance Schedule . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
Archiving and Backing Up SDS Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
Decontaminating the Sample Block . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
Cleaning Up and Defragmenting the Hard Drive . . . . . . . . . . . . . . . . . . . . . . . . . . . 114
Moving the 7300/7500/7500 Fast System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115
Monitoring Lamp Status . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
Replacing the Halogen Lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118
Replacing the Instrument Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
Updating the Operating System Software and Service Packs . . . . . . . . . . . . . . . . 124
Appendix B Creating a Background Plate 125
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125
Creating a Background Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125
Appendix C Creating a Custom Pure Dye Plate 127
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127
Determining Optimum Dye Concentration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128
Creating a Custom Pure Dye Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130
Adding the Custom Dye to the Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131
Appendix D Setting Up the Computer 133
Unpacking the Computer and Setting Voltage . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134
Connecting Components and Powering Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 136
Setting the Display Settings and Power Options . . . . . . . . . . . . . . . . . . . . . . . . . . 137
Connecting to the Network and Downloading Adobe Acrobat . . . . . . . . . . . . . . . . 140
Index 141

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)