PN 177196BB
7-6
DATA MANAGEMENT AND REVIEW
DATA ANALYSIS - AN OVERVIEW OF THE DILUTIONS AND MEASUREMENTS
Dilution Used to Determine the Hemoglobin Result
1. The final dilution made in the DIL1/HGB bath is used to determine the hemoglobin
parameter result.
2. The final dilution in the DIL1/HGB bath is prepared in three stages:
a. Primary dilution: 10 µL whole-blood is diluted with 1.7 mL of Diluent in the
DIL1/HGB bath to form a 1:170 dilution (commonly referred to as the first dilution)
b. Secondary dilution:
1) 42.5 µL of this first dilution is removed and 0.40 mL of Diluent is added.
2) 0.4 mL of Hgb Lyse reagent is added to form the final 1:250 dilution.
3. Hemoglobin released by lysis of the red blood cells combines with potassium cyanide to
form a cyanmethemoglobin compound.
4. Hemoglobin concentration is determined based on transmittance of light through the
optical part of the DIL1/HGB bath using a spectrophotometric technique at a wavelength
of 550 nm.
a. Transmittance of the sample dilution is compared to transmittance of a
reagent blank.
b. The Analyzer calculates Hgb using the blank and sample readings.
c. The right-side panel of the Analyzer prevents ambient light from interfering with
the Hgb measurements.
5. Table 7.2-2 summarizes the dilutions made and the measurement method used to obtain
the hemoglobin data. This same information is covered in
Table 2.3-3, Technical
Characteristics for the Measurement of the Hemoglobin
, in the Online Help System or the
Instructions for Use manual.
.
Table 7.2-2 Technical Characteristics for Obtaining Hemoglobin Results
Dilution Characteristics Primary Dilution for Hgb:
Volume of whole-blood 10 µL
Volume of Diluent 1700 µL
Primary dilution ratio 1:170
Secondary Dilution for Hgb:
Volume of primary dilution removed 42.5 µL
Volume of additional Diluent 400 µL
Volume of Hgb Lyse 400 µL
Final dilution for Hgb determination 1:250
Reaction temperature 35°C (95°F)
Measurement Characteristics Method of analysis Spectrophotometry
Wavelength 550 nm
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