PN 177196BB
7-7
DATA MANAGEMENT AND REVIEW
DATA ANALYSIS - AN OVERVIEW OF THE DILUTIONS AND MEASUREMENTS
7
Dilution Used to Determine the Reference WBC Count and the BASO Count
1. Two dilutions are required to obtain WBC count and differential parameter results:
a. WBC/BASO dilution
b. DIFF dilution
2. The final dilution in the WBC/BASO bath is used to determine the reference WBC count
and the BASO count.
3. The final dilution in the WBC/BASO is prepared by simultaneously delivering 10 µL of
whole-blood and 2.0 mL of WBC Lyse reagent into the WBC/BASO bath to form a 1:200
dilution.
4. The Analyzer maintains the reagents and reaction at a regulated 35°C (95°F).
5. The cells in the final 1:200 dilution in the WBC/BASO bath are counted and sized using
the Coulter principle.
6. The final 1:200 dilution inside the WBC/BASO bath is used to:
a. Determine the WBC count
b. Develop the WBC/BASO histogram, which is needed to obtain the BASO count.
7. The WBC count and BASO count are determined simultaneously.
8. When a CBC/DIFF panel is selected the WBC count is determined twice using two
different methodologies.
a. The count obtained in the WBC/BASO bath is the reference WBC count.
b. The second WBC count is determined in the flow cell during acquisition of the
DiffPlot. The dilution analyzed in the flow cell is prepared in the DIFF bath.
c. WBC count results from the two methodologies are compared and if the results
exceed predefined limits, the WBC count result is flagged.
9. Table 7.2-3 summarizes the dilutions made and the measurement method used to obtain
the WBC and basophil data. This same information is covered in
Table 2.3-4, Characteristics
Required to Obtain WBC/BASO Results
, in the Online Help System or the Instructions for Use
manual.
Table 7.2-3 Technical Characteristics for Obtaining WBC and BASO Results
Dilution Characteristics Volume of whole-blood 10 µL
Volume WBC Lyse 2,000 µL
Dilution ratio 1:200
Reaction temperature 35°C (95°F)
Measurement Characteristics Method of analysis Coulter Principle
Aperture diameter 80 µm
Count vacuum 200 mb (5.9 in. Hg)
Count period 2 x 6 seconds
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