CB (E7) 06/2018 Page 5/174
15. GENERAL INFORMATION ................................................................................ 116
15.1 Service contact page ....................................................................................................................... 116
15.2 Current operating parameters ......................................................................................................... 116
15.3 Event list .......................................................................................................................................... 117
15.4 Technical chamber information ....................................................................................................... 117
16. CHART RECORDER DISPLAY ......................................................................... 118
16.1 Views ............................................................................................................................................... 118
16.1.1 Show and hide legend .......................................................................................................... 118
16.1.2 Switch between legend pages .............................................................................................. 118
16.1.3 Show and hide specific indications ....................................................................................... 119
16.1.4 History display ....................................................................................................................... 119
16.2 Setting the parameters .................................................................................................................... 122
17. HUMIDIFICATION SYSTEM (CHAMBER WITH ACTIVE HUMIDIFICATION) .. 123
17.1 Function of the humidifying system ................................................................................................. 123
18. OPTIONS ............................................................................................................ 124
18.1 Communication software APT-COM™ 3 DataControlSystem (option)........................................... 124
18.2 Silicone access ports 30 mm / 1.18 in, closable from both sides with silicon plugs (option) .......... 124
18.3 Interior socket 230V (option, available via BINDER INDIVIDUAL Customized Solutions) ............. 125
18.4 Analog outputs for temperature and CO
2
(option) .......................................................................... 127
18.5 Data Logger kits (option) ................................................................................................................. 127
18.6 Access port for extra-low voltage (option) ....................................................................................... 128
18.7 BINDER Gas Supply Service – External bottle changer for CO
2
, N
2
or O
2
(option) ....................... 129
18.8 Stands ............................................................................................................................................. 129
18.8.1 Stacking stand (option) ......................................................................................................... 129
18.8.2 Stacking adapter for direct thermal decoupled stacking (option) ......................................... 129
18.8.3 Base on castors (option) ....................................................................................................... 130
19. REFERENCE MEASUREMENTS ...................................................................... 130
19.1 CO
2
reference measuring ............................................................................................................... 130
19.1.1 Measuring CO
2
concentration indirectly via the pH of the cell medium ................................ 130
19.1.2 Measuring CO
2
directly via chemical indicator tubes ........................................................... 131
19.1.3 Measuring CO
2
directly with an electronic infrared measuring device ................................. 131
19.2 Temperature reference measurement ............................................................................................ 131
20. AVOIDING MICROBIAL CONTAMINATION...................................................... 132
20.1 Cells and media .............................................................................................................................. 132
20.2 Laboratory conditions / equipment around the incubator ................................................................ 132
20.3 Working and behavior in the lab ..................................................................................................... 132
20.4 Chamber design and equipment of the CO
2
incubator ................................................................... 133
20.5 Handling the CO
2
incubator ............................................................................................................ 134
21. CLEANING, DECONTAMINATION / DISINFECTION, AND STERILIZATION .. 135
21.1 Cleaning .......................................................................................................................................... 135
21.2 Decontamination / chemical disinfection of the chamber ................................................................ 137
21.3 Hot-air sterilization at 180 °C / 356 °F............................................................................................. 138
21.3.1 Overview ............................................................................................................................... 138
21.3.2 Preparation for a hot-air sterilization ..................................................................................... 139
21.3.3 Starting the sterilization cycle and running the hot-air sterilization ....................................... 140
21.3.4 Prematurely terminating the sterilization cycle ..................................................................... 141
21.3.4.1 Prematurely terminating the sterilization cycle after less than 4 hours: Ineffective
sterilization .................................................................................................................... 142
21.3.4.2 Prematurely terminating the sterilization cycle after more than 4 hours, i.e., during the
cooling-down phase: successful sterilization ................................................................ 142
21.3.5 Completing the entire sterilization cycle ............................................................................... 143