Research Instruments INTEGRA 3 - Temperature Calibration Procedures

Secon 7
Integra 3™ Temperature Control

Research Instruments Ltd
7
Probe Posioned in Droplet Centred
over Objecve/Hole in Heated Plate
Probe Posioned in
Droplet over Heated Plate
Where the Thermosafe Air Heang System is ed, it is recommended
that temperature measurements are made above the centre of the
objecve and over the heated plate (see Figure 7-1 on page 35).
For opmum temperature control across the hole in the heated plate,
adjust the setpoint temperature of the Thermosafe Air Heang System.
To control the area directly above the heated plate, adjust the heated
plate setpoint temperature. As a starng point, use the same setpoint
temperature for both heang systems.
In all cases, it is recommended that the objecve normally used for
manipulaon is in posion when measuring specimen temperature and
adjusng setpoint temperatures.
Figure 7-1

Perform calibraon only if the displayed temperature is dierent to the
actual temperature of the heated plate surface or the air expelled from the
air heater nozzle. The process of calibraon allows the user to manually
adjust the temperature so that the displayed temperatures match the
temperature of the heated plate or air expelled from the air heater nozzle.
We recommend using a thermometer calibrated to 37°C ed with a
small thermocouple probe, such as the RI IVF Thermometer. Place the
probe of the thermometer in the centre of the water drop and allow the
temperature reading to stabilise. Adjust the setpoint temperature unl
the desired temperature in the dish is reached, allowing 20 minutes (or
as long as required) in between each setpoint change to allow the Petri
dish temperatures to stabilise.