Secon 5
Integra 3™ Basic Operaon
18
Research Instruments Ltd
5
Place the Petri dish in the centre of the insert, avoiding the frame of the
glass insert (gure 5-3). Short working distance objecves might touch
the insert before the specimen is brought into focus, depending on the
thickness of the glass and the dimensions of the Petri dish. Dishes with
a diameter larger than 60mm may need posioning by hand if the area
covered by specimen preparaons exceeds 40mm in diameter.
The moving stage plate is heated, allowing a dish to be posioned away
from the centre of the stage should this be required.
Place the Petri dish such that the area of interest is over the hole in the
centre of the insert. The eecve range of movement is limited by the
diameter of this hole (normally 25mm). Reposion the dish by hand to
view specimens that are outside the area of the hole. It may also be
necessary to reposion the dish if specimens are close to the edge of
the hole or if short working distance objecves are being used, as the
objecve might contact the insert.
Metal heated inserts are secured in posion using magnets, the insert
can be lied out by pulling upwards. When lowering the insert into
posion ensure that the insert is rotated unl the magnets re-aach the
insert securely.
The RI Droplet Organisaon Guide (Part Number 6-30-600) can be used
to posion droplets within commonly used Petri dishes in order to ensure
that all droplets are accessible by the pipees.
Exercise cauon when reaching for stage XY control that the
Fine Control Lever is not knocked, especially when switching
to the Integra 3 aer using other stages that have the stage
XY control in a dierent posion.