Research Instruments INTEGRA 3 - Equilibration Procedure

Syringes

Research Instruments Ltd
9
Secon 9

This procedure balances the pressure in the syringe with the capillary
acon of the pipee, allowing the most accurate control of the sperm
injecon.
The following is a typical procedure. However, other methods are
possible, eg using medium without PVP. Individual clinics may develop
their own procedures.
 Equilibrate with PVP
 Equilibrate with medium
1. Place a drop of PVP/medium in the centre of a Petri dish.
2. Ensure the rotator is at the lower end of the movement. Turn the
rotator down (clockwise), if necessary.
3. Press the E-buon (equilibrang buon).
4. Using the toolholder set-up lever, lower the micropipee into the
drop.
5. Rotate the SAS an-clockwise (ie upwards) 5 to 6 turns for an injecon
pipee, or 1 turn for a holding pipee. Do not allow the PVP/medium
to enter the tubing. The level of the PVP/medium should always be
clearly visible inside the pipee.
6. Press the E-buon.
7. Leave to equilibrate for 1-2 minutes.
E-buon
Figure 9-3