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5.15 Program 15: Durham
For liquids when the manufacturer recommends autoclaving at
temperatures of 121ºC (250F) for 15 minutes.
Cautions!
Both PT100 temperature sensors must be inside the bottles.
For proper sterilization, fill the bottles with approximately the
same amount of liquid.
Nominal parameters default settings
• Sterilization temperature: 121C (250F).
• Sterilization time: 15 minutes.
Operations sequence:
• (PV, pre-vacuum models only) Pulse low/Pulse high: at one pulse
it will build vacuum down to 25 kPa.
• Heating: Steam enters the chamber and heats it up until the
sterilization temperature is reached.
• Sterilization: Sterilization temperature is maintained constant during
the sterilization time.
• Slow Exhaust: Steam is exhausted from the chamber at a slow rate,
until chamber temperature reaches 105C.
• Cooling: Forced cooling to the required end temperature, chamber
pressure remains at approx. 140kPa, and the cooling valve is opened.
• Fast Exhaust: The steam is exhausted out of the chamber at a fast
rate until pressure decreases to ambient pressure.
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