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9.2 Tubing
• When placing tubing on the tray, make sure that both ends are open,
without sharp bends or twists.
9.3 Sterilization of Liquids
1. Use only heat-proof glass, filled 2/3 full.
2. Ensure that the glass container is covered but not sealed to prevent
pressure build-up.
3. Place the two temperature sensors into two separate liquid containers.
These are used to control the program temperature and ensure the
safety of the operating cycle.
Note: For isothermal cycles, insert one PT100 temperature sensor into the
liquid, and allow the other PT100 temperature sensor to hang loose
inside the chamber, (see the figure below).
For proper sterilization, ensure all the bottles are filled with
approximately the same amount of liquid.
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