A volume is the total signal intensity inside a defined boundary drawn on a lane. The
purpose of the volume of reference is to use volumes of known concentration to calculate
the unknown concentrations.
The volume of reference process follows the spot separation.
Note: you can either access the volume of reference function by clicking on the next
button of the background subtraction or directly by clicking on the volume of reference of
the 2-Spot Quantification folder.
The dashboard details the quantity of reference parameters:
The quantity of reference
The lane of reference
The spot(s) of reference
Q
UANTITY OF REFERENCE
The calculation of the unknown concentrations is based:
On the calculated volumes
On the known concentration. The known concentration is the quantity of reference.
The quantity of reference could correspond to one or several spots.
The purpose of the quantity of reference is to define the known concentration:
In the “Quantity of reference” edit field, type the quantity of known concentration you want
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