FIREREADER
FIREREADERFIREREADER
FIREREADER
SERIES
SERIESSERIES
SERIES
D
I S T A N C E C A L C U L A T I O N M O D U L E
Objectives and output
The FireReader 1D Distance module features the calculation of electrophoretic distances
according to markers or standards:
- in R.F. values (values between 0 and 1)
- in isoelectric points (pH units)
This function allows the assignment of RF values to the detected bands. To do so, an origin
line and a front line must be defined. Usually the origin is set to value 0 and the front to 1,
but the FireReader 1D allows you to set your own values for origin and front lines.
At the end of the process, you can have the following outputs:
- Profile and migration curve
- Calculated distance in between a front line and an end line.
The distance calculation process follows the band detection:
Note: you can access the distance menu by clicking directly on the distance tab.