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Note: only two positive or two negative images can be added. Thus, it could be necessary to inverse the image one or the
image two for having satisfactory results.
2- Then, select the subtraction image by clicking on “Select image” from the Second image
paragraph. A pop-up window displays the following menu:
A list displays the images already opened.
You can also add an image to the list by opening an image from your computer. To proceed, click on
the “From disk” button. A pop-up window displays the following menu:
Browse to specify the image directory
Double click on the image name you want to load
A new composite image appears in the main windows, subtracting one image from the other one.
3- Adjust the weight you want to give to each image
You can adjust the proportion of the marker and of the signals images in the composite image.
Example:
Cy3 image with some Cy2
Cy2 image
Cy3 image subtracted from
Cy2
To close the function, click on
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