UVITEC Cambridge FIREREADER MAX - Page 6

UVITEC – Cambridge 6/225
EXPOSURE MENU ......................................................................................................................................................44
Access to the Exposure modules ............................................................................................................................... 44
Auto-exposure ........................................................................................................................................................... 46
Manual exposure ...................................................................................................................................................... 49
Serial exposure .......................................................................................................................................................... 52
Focus point adjustment and motorized zoom controls ............................................................................................. 54
ANALYSIS MENU ........................................................................................................................................................56
Access to the analysis modules ................................................................................................................................. 56
M OLECULAR WEIGHT ANALYSIS MODULE ..........................................................................................................................58
Objectives and output ......................................................................................................................................................58
Molecular weight module (MW) operating environment ........................................................................................59
Toolbar in details..................................................................................................................................................................62
1 - D E T E C T ............................................................................................................................................................. 69
A – Edit lane ..........................................................................................................................................................................69
B – Edit band .........................................................................................................................................................................73
C – Add marker values ......................................................................................................................................................77
2 - A N A L Y S E – M O L E C U L A R W E I G H T ........................................................................................................... 82
A – Molecular weight results .............................................................................................................................................82
Return to the 1D acquisition module ........................................................................................................................ 83
C OLONY COUNTING ANALYSIS MODULE ............................................................................................................................84
Objectives and output ......................................................................................................................................................84
Colony counting (CC) module operating environment ..........................................................................................85
Toolbar in details..................................................................................................................................................................87
A U T O M A T I C C O U N T I N G .................................................................................................................................... 94
Detection ..............................................................................................................................................................................94
Return to the 1D acquisition module ........................................................................................................................ 95
M A N U A L C O U N T I N G ........................................................................................................................................... 96
Manual counting .................................................................................................................................................................96
Return to the 1D acquisition module ........................................................................................................................ 97
D ISTANCE CALCULATION MODULE ...................................................................................................................................98
Objectives and output ......................................................................................................................................................98
Distance calculation module operating environment .............................................................................................99
Toolbar in details................................................................................................................................................................101
1- D E T E C T ......................................................................................................................................................... 108
A – Edit lane ........................................................................................................................................................................108
B – Edit band .......................................................................................................................................................................111
C – Distance .......................................................................................................................................................................114
2 - A N A L Y S E – D I S T A N C E ............................................................................................................................... 117
A – Distance results ...........................................................................................................................................................117
Return to the 1D acquisition module ...................................................................................................................... 118
O PTICAL DENSITY 1D ANALYSIS MODULE ........................................................................................................................ 119
Objectives and output ....................................................................................................................................................119
Optical density / 1D (OD-1D) operating environment ............................................................................................119
Toolbar in details................................................................................................................................................................122
1- W I N D O W D E F I N I T I O N .............................................................................................................................. 128
A – Lane definition ............................................................................................................................................................128
B – Background subtraction ...........................................................................................................................................131
C –Spot separation ...........................................................................................................................................................136
2- A N A L Y S E – S P O T Q U A N T I F I C A T I O N ................................................................................................... 140
Principles of quantification .............................................................................................................................................140
A –Quantity of reference ................................................................................................................................................141
Return to the 1D acquisition module ...........................................................................................................................144
EDIT MENU .............................................................................................................................................................. 145
Access to the Edit module ....................................................................................................................................... 145
Text ......................................................................................................................................................................... 146
Crop ......................................................................................................................................................................... 150
Rotation .................................................................................................................................................................. 151
Pseudo color ............................................................................................................................................................ 152