1-6 Quick Reference Guide
Guidelines for Obtaining Samples
■ Serum samples: Use no-additive evacuated specimen collection tubes or serum
separator tubes.
■ Whole blood or plasma samples: Use only lithium heparin evacuated specimen
collection tubes.
■ Obtain a sample of approximately 100 µL.
■ When collecting the sample in lithium heparin collection tubes, fill the tube at least
half-way to maintain the proper ratio of sample to anticoagulant.
■ Run samples within 60 minutes. If this not possible, spin down and remove the
plasma or serum.
■ Place the plasma or serum into a clean red top tube, and run within five hours. If
this is not possible, refrigerate the sample in a stoppered container at 2–8 ºC
(36–46 ºF) for no longer than 48 hours. A plasma or serum sample can also be
stored at -10 ºC (14 ºF) for up to five weeks in a freezer that does not have a self-
defrost cycle.
Guidelines for Handling Samples
Use the following guidelines and procedures to ensure best results.
■ Before dispensing, remove the needle to avoid hemolysis.
■ Dispense the sample slowly into the sample tube.
■ For dehydrated patients: Use plasma, or allow the sample to settle for 5 minutes
and then draw from the top of the sample.
Syringe Sizes
■ Small breed: up to 10 kg — use a 25-gauge syringe.
■ Medium breed: 10–30 kg — use a 22-gauge syringe.
■ Larger breed: 30–60 kg — use a 20-gauge syringe.
■ Giant breed: over 60 kg — use an 18-gauge syringe.
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