1-8 Quick Reference Guide
■ Syringe pre-wetted with lithium heparin.
Make sure the heparin concentration is d 15 units per mL of blood.
a. Expel excess heparin by moving the plunger back and forth several times,
until only a fine coating is left inside the syringe barrel. This is important for
preventing excess sample dilution, which will alter final sample results and
can cause errors.
b. Change the needle to make sure all excess in the needle hub is removed.
(A new needle is also sharper.)
c. Draw the sample.
d. Mix the sample well by gently inverting two or three times.
Step 3: Add Sample to the Rotor
Sample Delivery Volume
Follow these guidelines to make sure you add the correct sample volume to the reagent rotor.
■ When a sufficient quantity of sample is added,
it will form a line between the arrows on the
rotor surface.
■ If an air bubble is introduced, add enough sam-
ple to fill the overflow area by 1/3 to 1/2, as
shown at right.
■ Make sure any excess sample does not travel
beyond the overflow area.
90 µL
> 90 µL
< 120 µL
<90 µL
> 120 µL
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