Quick Reference Guide 1-9
Using a Fixed 100 µL Volume Pipette
1. Firmly attach a new tip to the end of the pipette. Do not touch the tip, or a false elevation
of amylase can result.
2. With your index finger or thumb, push the pipette plunger to the stop
position, and hold it there for sample pickup.
3. Immerse the tip 2–3 mm below the surface of the sample, as shown at
right.
4. Slowly release the plunger to draw the sample. Pause, then remove the pipette from the
sample tube.
5. Make sure there are no air bubbles or gaps in the pipette tip.
6. Place the pipette tip into the rotor’s sample port, so that the tip just
touches the sample port, as shown at right.
7. Gently and slowly depress the pipette plunger to allow sample to
slowly fill the sample chamber. See “Sample Delivery Volume” on
page 1-8 for the appropriate sample delivery volume.
Note: Keep the pipette plunger pressed down until you remove the tip
from the sample port.
Using a Disposable 100 µL Capillary Pipette
1. Keep the capillary pipette horizontal until it is over the sample
port.
2. Raise the pipette to vertical (so it is perpendicular to the rotor
surface), and place its tip in the sample port, as shown at right.
3. Push the black plunger down slowly and smoothly until the
white plug reaches the end of the capillary pipette. (There will be a slight resistance at
first.)
See “Sample Delivery Volume” on page 1-8 for the appropriate sample delivery volume.
To Next Page
Loading...
