Abbott AEROSET - Page 49

PROPRIETARY INFORMATION
CONFIDENTIAL MATERIAL
Not for Customer Distribution Assays/Reagents
AEROSET
®
System Troubleshooting Guide 49
94816-107—November 2004
ETOH
ADH
Ethyl Alcohol + NAD NADH + acetaldehyde
ADH = Alcohol dehydrogenase
NADH = Nicotinamide adenine dinucleotide
Uses Ethanol 0 and
100 mg/dL calibrators
for calibration.
Sample handling:
ethanol is a volatile
analyte. To prevent
evaporation, ethanol
sample containers
must be kept tightly
capped.
GGT
L-γ-glutamyl-3-carboxy-4-nitroanilide + glycylglycine
L-γ-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
The Roche GGT assay
on the
Roche/Hitachi 717
has approximately a
30% low bias
compared to the
AEROSET System.
Abbott has
documentation to
support this bias and
the accuracy of the
AEROSET values. The
AEROSET
methodology utilizes
the IFCC method.
Glu
Glucose + ATP Glucose-6-Phosphate + ADP
Glucose-6-phosphate + NADP
+
6-Phosphogluconate + NADPH
HK = Hexokinase
G-6-PD = Glucose-6-phosphate dehydrogenase
Hapt
Haptoglobin + PEG/buffer (R1) + Antibody to haptoglobin (R2)
Turbidity due to Haptoglobin:Antibody complex
PEG = Polyethylene glycol
Assay
Name
Reaction Tips
* MULTIGENT
®
assay
GGT
HK
G-6-PD