Abbott AEROSET - Page 50

PROPRIETARY INFORMATION
CONFIDENTIAL MATERIAL
Assays/Reagents Not for Customer Distribution
50
AEROSET
®
System Troubleshooting Guide
94816-107—November 2004
HbA1c*
Total Hemoglobin:
Hemoglobin + Alkaline Non-ionic surfactant
Hematin (green solution)
Hemoglobin A
1c
:
HbA
1c
+ Anti-HbA
1c
Antibody:Microparticle complex
HbA
1c
:Antibody:Microparticle complex
Remaining unbound Anti-HbA
1c
:Antibody:Microparticle complex +
HbA
1c
Hapten HbA
1c
Hapten:Antibody:Microparticle complex
HbA
1c
concentration
is inversely
proportional to
absorbance change.
(High absorbance
change = low HbA
1c
concentration; low
absorbance change =
high HbA
1c
concentration.)
HDL
HDL, LDL, VLDL, Chylomicrons Stable complexes
HDL HDL Disrupted
HDL Cholesterol ∆
4
Cholestenone + H
2
O
2
H
2
O
2
+ DSBmT + 4-AAP Quinoneimine Dye + H
2
O
DSBmT = N,N-bis(4-sulphobutyl)-m-toluidine-disodium
4-AAP = 4-Aminoantipyrine
Linearity and control
material must be
human-based
material.
IgA
IgA + PEG/buffer (R1) + Antibody to IgA (R2) Turbidity due to
IgA:Antibody complex
PEG = Polyethylene glycol
IgG
IgG + PEG/buffer (R1) + Antibody to IgG (R2) Turbidity due to
IgG:Antibody complex
PEG = Polyethylene glycol
IgG may be very
elevated due to an
IgG monoclonal
paraprotein.
IgM
IgM + PEG/buffer (R1) + Antibody to IgM (R2) Turbidity due to
IgM:Antibody complex
PEG = Polyethylene glycol
Samples containing
high IgM can have
increased viscosity.
Assay
Name
Reaction Tips
* MULTIGENT
®
assay
Detergent
Polyanion
Detergent
Cholesterol Esterase
Cholesterol Oxidase
Peroxidase